Tag

Part:BBa_J70044

Designed by: Julie Norville   Group: Knight Lab   (2009-01-22)

6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA

This 6 His suffix tag can be used with BBa_J70010 (PpiI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.


Oligos needed for annealing the part and placing it into a XbaI/PstI cut vector, possibly pSB2K4 that contains J70010 or J70030:

Forward: ctagagatcccaccatcaccatcatcattaatactagtagcggccgctgca

Reverse: gcggccgctactagtattaatgatgatggtgatggtgggatct

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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